Sounds like you might have leaky bottles. Also be very careful with any extremes in temperatures. Yeasts are living things and as with many organisms, extreme or rapid changes in temperature can shock yeasts.
Another thing, the length of tubing can make a difference, but only a very small amount and only with very very long lengths. So seeing your lengths I do not think it is that.
I agree with genghis on the tubing. The harder polypropylene type like you are using does not seal as well as the soft sqwishy green silicone type. But I disagree with the check valve issue he mentioned. The check valve is to prevent siphoning yes, but mainly its about maintaining consistant pressure in the system.
Just my 2c
Ummm ... Junior member. Man I have title envy!
