05-26-2018, 04:37 AM
Join Date: Apr 2018
| | Re: Lab journal - tissue culture startup
Liquid media test I started 2d ago seems to be working well. Had some plants come in that were contaminated in their tissue culture packaging already so I had to try to save them. Lost one completely, nbd. The others made it into a quick isopropyl wash, bleach wash, rinsed, and into my experimental media before their new cubes got completely overtaken by bacteria and fungus. The 4 I was worried about all turned out nasty. Lost one from that batch though. Also decided to take two cups I was to throw away, Glossostigma, and try to save em. Original and replacement were both horribly bloated and slimy with bacteria. Same lab as the others.... No surprise. Different website but same supplier. Rather than toss em I hit em with a little more aggressive steralizing protocol, and before work this morning they also got put in my low agar liquid media on the orbital shaker. Time will tell. Glosso is cheap enough, but it'll be a good test. If I can save it, odds are good that protocol will work on the plants coming out of my aquarium next. Submerged (and terrestrial plant explants taken from under soil) plants can be hard to steralize without killing them. So this is as much a test of the media and protocol as it is saving 2 cups of glosso. In a week I'll move them back to a more solid media on the grow racks with the rest. Got all my tools and glassware cleaned last night so I'm ready to get back to multiplying tonight after work! Aiming for another dozen or so.
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