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DIY Aquarium Projects For those that are handy or looking to save some money, discuss your DIY aquarium projects here.

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Old 05-25-2018, 06:03 PM   #21 (permalink)
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Default Re: Lab journal - tissue culture startup

I sold the last of my penny stocks today. So in a few days when the cash is reconciled, and then once I GET the cash..... I'm likely buying all the above species I can afford. Got about $600 coming. So I should be able to buy every single thing from Ukraine and a ton from my Indian contact. I'll need a second stock tank before long haha

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Old 05-25-2018, 06:07 PM   #22 (permalink)
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Shiz where are you selling? XD i need in this!
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Old 05-25-2018, 06:08 PM   #23 (permalink)
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Selling plants or stocks? Stocks was NYSE via Fidelity. Plants.... I don't have anything available for sale yet. About a third of current stuff in culture multiplying tho

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Old 05-25-2018, 06:10 PM   #24 (permalink)
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Default Re: Lab journal - tissue culture startup

OOHHH i thought you meant plant stock
i remember you telling me about the stock thing nvm lmao sorry.
When you get ready to sell remember to hit me up early.
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Old 05-25-2018, 06:26 PM   #25 (permalink)
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Default Re: Lab journal - tissue culture startup

I bought some cultured Java ferns a few months back from petsmart. I put it in my low tech tank. It doesn't grow new leaves except for baby plantlets under its old leaves. Is this normal?
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Old 05-26-2018, 12:49 AM   #26 (permalink)
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Did you bury the roots? They don't appreciate that

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Old 05-26-2018, 12:54 AM   #27 (permalink)
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I have some in my low tech ATM. Was a small chunk of windelov. I anchored it by putting between two small fake granite caves. There was 3 caves and some rocks grouped together. Only been in there about 8 or 9 months, and there's a 6 inch mat of windelov where it has just overgrown and overtaken everything. If you burried the rhizome, try anchoring it with fishing line, superglue, between deco, etc. It will grip on to whatever it is against and go crazy. They pull most of nutrients from water column not soil. Grow real slow (or die when rhizome rots) if you bury the bottom

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Old 05-26-2018, 03:37 AM   #28 (permalink)
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5/26
Liquid media test I started 2d ago seems to be working well. Had some plants come in that were contaminated in their tissue culture packaging already so I had to try to save them. Lost one completely, nbd. The others made it into a quick isopropyl wash, bleach wash, rinsed, and into my experimental media before their new cubes got completely overtaken by bacteria and fungus. The 4 I was worried about all turned out nasty. Lost one from that batch though. Also decided to take two cups I was to throw away, Glossostigma, and try to save em. Original and replacement were both horribly bloated and slimy with bacteria. Same lab as the others.... No surprise. Different website but same supplier. Rather than toss em I hit em with a little more aggressive steralizing protocol, and before work this morning they also got put in my low agar liquid media on the orbital shaker. Time will tell. Glosso is cheap enough, but it'll be a good test. If I can save it, odds are good that protocol will work on the plants coming out of my aquarium next. Submerged (and terrestrial plant explants taken from under soil) plants can be hard to steralize without killing them. So this is as much a test of the media and protocol as it is saving 2 cups of glosso. In a week I'll move them back to a more solid media on the grow racks with the rest. Got all my tools and glassware cleaned last night so I'm ready to get back to multiplying tonight after work! Aiming for another dozen or so.

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Old 05-26-2018, 05:52 AM   #29 (permalink)
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Default Re: Lab journal - tissue culture startup

Quote:
Originally Posted by adkaqua View Post
Did you bury the roots? They don't appreciate that

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No, I stuck them on a driftwood. They're windelov and narrow leaf.
I wonder if it's a transitional thing where they were grown emersed and can't adapt to submerged except to grow plantlets.
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Old 05-28-2018, 12:16 AM   #30 (permalink)
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Might be. Haven't had that problem myself. Might not like your water. Might be a lot of things. I'd have to research it myself

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