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Old 10-22-2019, 03:36 PM   #1 (permalink)
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Default Tissue Culture Plants - How to Use them

For a few years now I have been looking for a good beginner's article about how to use tissue culture plants. Those tiny plants have even smaller roots, and are very fragile to handle. But, I still want to learn to use them. Who wants to give us a detailed post on how to use them and succeed? (I already have discovered ways to use them and fail.)
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Old 10-25-2019, 06:33 AM   #2 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

Following because I also seek this wisdom.
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Old 10-25-2019, 07:56 AM   #3 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

Here's how tropical does it. They go from lab grown to greenhouse (grown emerged).
https://www.youtube.com/watch?v=tsZmYl9CsPc

You can go from lab grown to aquarium though. I don't know what plant or setup the OP has.
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Old 10-25-2019, 12:00 PM   #4 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

If you are setting up a new tank, I can see it being possible to plant those minute plantlets, then carefully adding a little water and waiting for them to start growing. But, in an established tank I haven't had any luck thinking how to plant them. They are well below the size my fingers can handle.
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Old 10-25-2019, 01:06 PM   #5 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

To be honest, that company shouldn't sell something so delicate. They should let the plants develop longer roots at least.

If I have something so small, I would take the mat put in on wet dirt to develop/mature more.
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Old 10-26-2019, 08:41 AM   #6 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

If anybody who knows about tissue plants, it's @adkaqua
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Old 10-26-2019, 08:27 PM   #7 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

Hah. Thanks. I like to think I do, but sometimes you gotta just wing it.



As for planting ... There's 3 route.

First is for terrestrial plants. Things like orchids. Plant them in your soil. Keep the humidity at 100% with something like a sandwich bag hooked around the top with a rubberband. A week later cut a little slit in bag. Give a few days, even a week or so, enlarge slit a little. Repeat. Repeat. Till bag is cut open, then remove bag. This is called acclimatization, or hardening off. Gives the plants a chance to grow new leaves with a cuticle layer and functioning stomates to help prevent water loss,

Second is for things like terrarium plants. They're gonna be in high humidity. Just plant them and keep the humidity up. It'll be like living in that closed plastic bag forever anyways.

Third .. aquatics ... So this can be tricky. If the plants are grown on a solid media, they're basically emersed. Same as... Say ... The sword plant you bought potted at PetSmart. They were grown in a greenhouse at almost 100% humidity. Tissue culture grown on a solid media is growing in similar conditions. They'll still melt a bit. You can dry start them in a high humidity tank. Cover it with cling film. Then flood once roots grow good and plant is established. It'll melt, but at least it'll have the root system to grow back from. Or you can just plant them straight up in your tank, submerged. Roll the dice. Mosses will prob be fine. Other things can be more sensitive. If it's grown submerged in a liquid media... it'll basically already be in that submersed form. Shouldn't melt much at all. Afaik.... I'm the only one producing and selling plants like that though. Majority of your TC plants are basically sterile emersed plants. If you plant them just straight up in a tank... Supplementing CO2 with a high tech system (NOT LIQUID CARBON), and ensuring high lighting can help them more quickly adapt. There's far far far less available O2 and CO2 when submerged. So ensuring good gas exchange with water circulation and CO2 supplementation can go a long ways. Even if you only run high tech with a little off the shelf kit with a disposable cannister while establishing the plants... It'll help them make it through to growing submersed leaves that can more readily handle gas exchange underwater.

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Old 10-26-2019, 08:40 PM   #8 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

Thanks adkaqua. If I am planting stem plants, I know how to poke them into the substrate with tweezers. If I am planting rooted plants, I know a couple of ways to poke them into the substrate and get the roots under the substrate, but not to far under. But, when I try to plant these tiny plantlets I can't use tweezers without crushing them, and they are too small to handle with fingers, and the mechanics of getting them into the substrate without damaging them seems impossible. Has anyone seen a Youtube video showing them being planted into the tank?
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Old 10-26-2019, 09:33 PM   #9 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

The only plants I can think of that’s that small is HC, baby tears. People usually plant those in little clumps instead of individual plant.
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Old 10-27-2019, 07:10 AM   #10 (permalink)
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Default Re: Tissue Culture Plants - How to Use them

I bought a little container of tissue cultured plants from my LFS a couple of years ago, but I don't recall what plants it was. I ended up just tossing them. And, at about the same time I bought a container of them from an online seller, and did the same thing, but again I don't remember what plant is was. Last night I found a YouTube, https://www.youtube.com/watch?v=blTvsOevK2k, by Dennerle, but the cup of plants were way bigger than any I see in stores here.
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