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Old 03-07-2013, 05:51 PM   #11 (permalink)
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Default Re: TC experimentation

I'm not trying to suggest Neil shouldn't try out the Rotala first, just that after a few experiments to get the hang of it, I don't really see the point in continuing to do so when there are so many other plants that make for a more worthwhile result for all that effort... those Anubias sp. 'stardust' sound like a pretty cool idea to me!

TC's definitely not necessary for even large commercial-scale propagation, though. I've observed commercial stem growing facilities in action (admittedly, they were for herbs instead of aquatics, but the same principles apply)—they use cuttings and dirt. I've absolutely no doubt that if I wanted to, I could generate a few thousand stems of a species in a year using conventional means. I just don't know what I'd actually do with all of them afterwards LOL.
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Old 03-08-2013, 08:13 AM   #12 (permalink)
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Default Re: TC experimentation

Frank W. commented on TPT that anubias are not able to be propagated by TC because they coexist with a beneficial bacteral species and hence, are not able to survive the sterilization step. I have no idea if this is true or not but just thought I'd let u know what he said and let u figure out if it's true or not.
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Old 03-08-2013, 01:25 PM   #13 (permalink)
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Default Re: TC experimentation

Quote:
Originally Posted by JeffyFunk View Post
Frank W. commented on TPT that anubias are not able to be propagated by TC because they coexist with a beneficial bacteral species and hence, are not able to survive the sterilization step. I have no idea if this is true or not but just thought I'd let u know what he said and let u figure out if it's true or not.
I've actually been talking to some Asian growers and propagation of Anubias/bucephalandra species is definitely viable. check out this link, they offer anubias varieties in TC. e.g. anubias gabon, anubias butterfly, etc.
http://translate.google.com/translat...com%2F&act=url
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Old 03-09-2013, 06:32 PM   #14 (permalink)
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Default Re: TC experimentation

I have heard something vague about aroids requiring a different growth hormone than other cultures, but nothing about a bacteria (if they need it to coexist, for that matter, why would it only be on the outside of the plant material?)

I do know that many orchids require the presence of this or that microbe to germinate, but that's only when you try to grow them from seeds/seed material. TC using other parts of the plant works just fine.
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Old 03-09-2013, 07:20 PM   #15 (permalink)
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Default Re: TC experimentation

Quote:
Originally Posted by JeffyFunk View Post
Frank W. commented on TPT that anubias are not able to be propagated by TC because they coexist with a beneficial bacteral species and hence, are not able to survive the sterilization step. I have no idea if this is true or not but just thought I'd let u know what he said and let u figure out if it's true or not.
Absoltely NOT true. Tropica considers Anubias their "green gold" because they can propagate it so much faster using TC. Frank has this one completely wrong.

I can show you all photos of Anubias in TC at Tropica (and elsewhere) if anyone is intersted... Just not from my iPad, so it will have to wait until I'm back on my desktop computer.
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Old 04-06-2013, 07:28 PM   #16 (permalink)
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Default Re: TC experimentation

made the medium today!
since it was my first time i wasn't really sure about much and may have screwed up here and there but all in all i think i did pretty well.




some things i did wrong, so apparently for subculture you need 2 tablespoons and i didn't notice that at first so i thought it was 1 tablespoon oh well :/ i cut the recipe down by half because 1. don't have that many jars and 2. don't want to use all of it and screw up on the first try. so i'm guessing i put about 0.7-8 tablespoons of sugar? ehehe

secondly.... the instructions say to add food coloring first, but since i was using ph strips i realized i probably should have put it AFTERWARDS since the light tint may have screwed with the colors.

and one thing i didn't consider was what if the ph of the solution was WAY over or under the range the ph strips showed? since im using narrow range ph strips i don't think i'd be able to tell. oh well fingers crossed! hopefully this works!

btw... im using this on TC elatine hydropiper i got from frank at ADG so for now im just doing subculture. this TC stuff is so strenuous! >

and last thing was i gave my pinch, smidgen, dash spoons away to someone couple months ago because i figured i wouldn't need them LOL well I didn't want to go buy another set and it wouldn't arrive on time anyways so i just used my thumb and index finger for a "pinch"
there are alternatives to agar however, you can use sand that's been boiled or you can use coffee filter paper. the main thing is for the plant to not drown in the medium and it should be supported while having contact to the nutrients.
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Old 04-06-2013, 09:19 PM   #17 (permalink)
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Default Re: TC experimentation

Here's a bit of reality in this hip indeavor:

- Turns out tissue culture plants often have issues adapting to the real aquarium world.

- Tissue culture aquarium plants are of interest for one single reason - they last a few weeks on the store shelf and look cool doing it.
http://www.tropica.com/en/about-trop...aboratory.aspx


Other than that - yes it is fun to make 1 out of 20 jars actually grow a plant clean from diseases/fungus/bacteria.
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Old 04-06-2013, 10:00 PM   #18 (permalink)
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Default Re: TC experimentation

well it's worth a shot and im playing with anubias linea cup elatine hydropiper so it should be easier. plus it doesn't cost that much to do it and i think the experience is worth it.
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Old 04-07-2013, 10:42 AM   #19 (permalink)
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Default Re: TC experimentation

Quote:
Originally Posted by niko View Post
- Turns out tissue culture plants often have issues adapting to the real aquarium world.
I've had no issues with this myself. I've grow Rotala macrandra 'Japan Red', Hygrophila pinnatifida, Elatine hydropiper, Crypt. 'Flamingo' and Alternanthera reineckii 'Mini' all from commercial tissue culture. I'd say most took about as long to convert as if I'd grown them emersed first.
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Old 04-19-2013, 08:27 PM   #20 (permalink)
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Default Re: TC experimentation

Elatine hydropiper has been initiated into the media for a week now! They are starting to perk up! Fingers crossed for growth!
on a side note, the elatine i put into my emersed setup didn't do too well, though there are some stems that have clung on to dear life LOL


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