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Old 05-04-2018, 03:34 PM   #61 (permalink)
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Default Re: TC experimentation

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Originally Posted by hoppycalif View Post
I agree that resurrecting an old thread can be very productive. Just don't expect the person(s) being questioned are still monitoring this.
If you mean the OP i have with no doubt they are not seeing this anymore lol
As for the guy who bumped this, he is in the discord server i started and has a Huge TC production going on, with actual equipment etc, ive never seen anything like it.
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Old 05-04-2018, 03:52 PM   #62 (permalink)
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Default Re: TC experimentation

See now they're gonna go to discord. See my Warcraft name. And then just think of me as a scrub mage with garbage mythic parses. Gg.



But yea, I'm figuring I'm a few weeks out from starting. Went to school for this stuff. Sick of being in semiconductor industry. So I took half my 401k out. Building my own lab. Only thing that's proving problematic to find cheaply that will ship to residential address is hormones.


You can't even buy cellulase for making protoplasts without a business address. I'm working out of a 2br apartment to start. So that's our until I'm cash positive and can afford real dedicated space. Saw this on lunch today and thought maybe someone has done some small scale TC work for ornamentals or aquatic plants and might share source for reagent grade growth regulators. I'm prob gonna hit pot forums too. See what the biologists over there have found reliable and affordable

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Old 05-04-2018, 04:13 PM   #63 (permalink)
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Default Re: TC experimentation

It's mostly actual equipment. Little diy. I've got a Barnstead still with collection jug. Biosafety cabinet (overkill, but got cheaper than laminar, and it is just a laminar cabinet with extra features protecting user AND samples). Analytical balance. Hotplate stirrer. 10/100/200/1000uL pipettes. Accumet pH meter. Magenta TC vessels (glass and plastic).

But also things a utility bench for lab bench. 14qt electric pressure cooker for a stand in autoclave. Panty rack with 4 bulb t5 HO fixtures wired over each shelf for grow space.


Still need a mini fridge and dessicator for chems like MS salts. Microwave for media prep that hasn't had food cooked in it. Pipette tips for everything but 1000uL. New pH probe. Hormones. Basal salts. Other media supplements.

And about $600-800 in plants I plan to use not only in culture to start operation but to fill out the massive overhaul of a 35g tank I'm also doing simultaneously (new seals, internal sump with fluidized bed, handmade 3d background with sand fall, diy co2 reactor, fugeray2 & planted+ 24/7 on custom acrylic risers on top of tank after I remove rim and replace with euro, oh... And co2. Never used that before. So it's getting co2 tank, controller, and I'm scaping the whole thing with Dennerle soil).


Needless to say my apartment is craaaaaazy disorganized rn. Plumbing testing in bathroom. Foam and concrete work on kitchen table. Spare room becoming lab and grow space. All while working 12.5h shifts in semiconductor foundry, and keeping up with a 3 day raid schedule in mythic guild (#9 dps dealing mage on the server). Things are a bit crazy. And old threads like this with existing responses not only can pull ppl from previously back in to convo with experience in the topic at hand, but have comments already and are more likely to bring in NEW voices to join what they see as an existing convo.

Plus saves time to hit reply rather than back out in this app, start new, title, review it all for spelling and grammar and autocorrect making you look racist or like a jerk or swapping in swear words inappropriately as the op.... >.>

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Old 05-04-2018, 04:34 PM   #64 (permalink)
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Taped up thing is blocking off for epoxy/sanding/painting co2 reactor. Hex tank is hotel for fish, not cycled yet. Little stand has Xmas moss in an emersed experiment. Concrete foam thing is part of backdrop, with integrated sandfall. So my original necro wasn't a random act of 'whatev' lol

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Old 05-04-2018, 04:37 PM   #65 (permalink)
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Default Re: TC experimentation

Just reading all that, i should have a PHD.
you really gotta start a journal on that, if you didn't, then... well... IDK WHAT I'D DO.

But for most people including me this is a Must See operation your doing here and no one else i believe has documented something like this *updated*
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Old 05-04-2018, 04:47 PM   #66 (permalink)
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Default Re: TC experimentation

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But for most people including me this is a Must See operation your doing here and no one else i believe has documented something like this *updated*
I think you're prob right. I get some nods and such, but I think most ppl are like 'meh. Internet. Pretending to be someone or do something but never gonna do it'. I don't think people realize it's not just blustering lmao.


Even my wife didn't take it too serious. I had the capital equipment .. but still. Till I took my retirement out and bought the last stuff and boxes and boxes started coming. .. I don't think even she believed me.


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Old 05-04-2018, 06:04 PM   #67 (permalink)
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Default Re: TC experimentation

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I think you're prob right. I get some nods and such, but I think most ppl are like 'meh. Internet. Pretending to be someone or do something but never gonna do it'. I don't think people realize it's not just blustering lmao.


Even my wife didn't take it too serious. I had the capital equipment .. but still. Till I took my retirement out and bought the last stuff and boxes and boxes started coming. .. I don't think even she believed me.


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Well i wish you good luck

oh and dont forgot to make a journal PLZ xd

-Nate
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Old 05-05-2018, 01:25 AM   #68 (permalink)
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I haven't even been using shopping lists hahahah. Just running tally in my head.


If this is successful and I become a commercial seller, my brother is going to come on with me and build freshwater aquariums, terrariums, and vivariums. That .... I'll def document. This, I'll make a thread when I actually go live after my last shipment comes in. Post status updates and chat with people and such. Find out what plants there's overwhelming demand for and no supply. Teach where I can. Hell maybe hire if they're the right person. 1 lab tech can only handle 50, maybe 60k plants a year alone. I'm hoping to go well beyond that. What better place to find people as passionate about not only plants, but specifically aquarium plants that would want to join on. Hah. There'll be a thread. I'll throw it in disc when I'm going live and keep it updated

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