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the needle valve should be grippable from the bottom. I had to replace mine and i was able to do it with the regulator on the tank and it seals fine. (i only did a new needle valve as my old was gunked up).

If you take channel locks and grip the sides of the needle valve there are 2 flat areas, The bite of the channel locks should go there. Make sure your twisting top to the left or your gonna work against yourself.
 
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