You need to readjust the regulator every time you refill the CO2 tank. When you refill it, you remove the regulator assembly from the tank, and to do that, first you back off the adjustment knob until it is loose. Then you close the valve on the tank and remove the regulator. When you have the tank filled and are ready to install the regulator again, first you screw the regulator onto the tank, then, making sure the adjustment knob is backed all the way off, you open the tank valve. Now, you tighten the adjustment knob until you get about 20 psi on the output gage. If you didn't touch the needle valve before you removed the regulator you should be back at the bubble rate you want.
But, until the tank of CO2 warms up to room temperature you have to keep tightening the adjustment knob every half hour or so to get the pressure back up to about 20 psi. This is a very cheap regulator, that is only good for maintaining a constant output pressure if the input pressure is also constant. Good regulators will hold the same output pressure for any inlet pressure. But, good regulators cost a lot more too.