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The same result can come from a leak at any hose connection. I wrap each connection with wire and twist to ensure a leak proof connectiion. Then I check every connection with a water/detergent bubble test (use a brush to apply) to be sure no bubbles form.

I have had a 20# cylinder go dry in 1 week from a little leak at a hose barb.
You do not need teflon tape. The plastic or fiber washer you can get at the filling place is supposed to fully seal the main joint at the butt joint (flange face). Taping the threads does not seal the area around the neck of the regulator and the nut and can actually encourage leakage as you can't tighten the flange contact area as much.

Steve Pituch
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