Some questions on Tissue Culture

PPM - Parts Per Million

In the APC Library you can find a transcript of a chat presented by Art on Tissue Culture

Thanks for you reply, gnatster... in this case PPM is not part per million.... i found the meanig into the kitchencultirekit site, it is Plant Preservative Mixture, a product to sterilized the growing media.

That transcription is quite tedious for me, my english level is so poor...

Any practice case?.... maybe HeyPK?

Any "homemade" alternative to PPM?

Xema said:

Thanks for you reply, gnatster... in this case PPM is not part per million.... i found the meanig into the kitchencultirekit site, it is Plant Preservative Mixture, a product to sterilized the growing media.

That transcription is quite tedious for me, my english level is so poor...

Any practice case?.... maybe HeyPK?

Any "homemade" alternative to PPM?

Click to expand...

Hi there
There is a TC mailing list available.
Send a mail to: [email protected] with subscribe in the subject or body of the mail (try both as I can't remember which). There are many folks from all over the world on this list. They will better inform you of where to buy equipment and such in your area. Good luck

Xema,

If you wish to try some Plant Preservative Mixture, I have 30ml sitting in cool storage (about 3 months old). The one and only supplier charges USD1.00 per ml. I can mail you the sample providing you pay the mailing charge (or do a trade of some type). Please PM me if interested.

Andrew Cribb

Before an overseas shipment i will try get some sustitutive for PPM, maybe in that mailing list thare are people from more near countries.

Thanks!!!

Thanks for the reply.

Andrew Cribb

Hola Xema

Saludos, yo estoy en Mexico y tambien he intentado el cultivo celular ( soy laboratorista y tengo experiencia con cultiuvo celular pero de animales, la tecnica es totalmente diferente), los medios son efectivamente dificiles de conseguir, he intentado sustituirlos por fertilizantes de uso agriciola con regulares resultados, mi problema recurrente es la contaminacion con hongos, esto a partir del tejido, la esterilizacion con cloro y alcohol no me funciona muy bien (mis placas testigo siguen esteriles), por alli encontre que el cloro de alberca funciona mejor, espero tener algo de tiempo para probar con esto.
Ojala y pudieramos mantener el contacto

Saludos
Ruben Echeveste

ups I'm sorry this was a PM but I got confused with the buttons

Hola, Ruben

Intenta la esterilizacion con una disolucion de Hipoclorito Sodico al 1% y una gotas de jabon para platos

Mi problema es la esterilizacion del medio de cultivo, y la introduccion del material vegetal en los medios de cultivo en un entorno esteril.

English:

Try sterilization with a 1% sodium hypochlorite disolution and some drops detergent.

my main problem is get sterilized medium, and putting vegetal stuff in medium under strilized atmosphere.

Sorry for being late to this.

You don't need PPM to do tissue culture. It is a relatively new anti-fungal chemical designed to reduce your losses. With proper sterilization, you don't need it.

The foremost expert on this topic is Dr. Michael Kane of the University of Florida. He has written several articles on this that you may be able to get. I was successful in tissue culturing common Cryptocoryne following his techniques.

If you are interested in this area, I highly recommend that you purchase Plants from Test Tubes: An Introduction to Micropropagation. It will walk you through the equipment you need, how to setup a work area, and the stages of tissue culture. The book is truly the bible for tissue culture.

Let me know if you have any other questions on this topic.

Saludos,

Art, have you tried this on stem plants? Is this a better and faster substitute to reproducing plants?

Raul,

I assume you mean reproduction by cutting or runner. There is no doubt that micropropagation will lead to many more plants than any other method. That is why nuseries are moving to this form of growing. So, if you ask, "What is the fastest way for me to get 1,000 clones of X plant without having to purchase 1,000, the answer is micropropagation (tissue culture). In addition, microporpagation will result in disease-free, strong plants. This is not always the case using other methods.

However, micropropagation is very difficult to do without a) a knowledge of the protocol for each plant and b) the right equipment. As a result, it is harder to do than other methods. This is why not all nusery plants are grown this way.

So, based on the above, I'm not sure it's better. It depends on your objectives, time and cash.

Xema,

I'd be interested to follow your project. There is a name (that I can't remember right now) for plants that can be cloned from leaf parts. Unfortunately, most aquatic plants don't fall within that group of plants. Therefore, you need to go get good clean apical meristems from shoots. These are the cells growing at the tip of the shoots. They are your best bet in getting good clones.

About how much money did you spend on your setup Art? This sounds like an intriguing endeavor down the road.

I think I was about $75-$100 into it when I got started. The costly part is when you start to grow out plants. You need to keep them somewhere!

Art_Giacosa said:

I think I was about $75-$100 into it when I got started. The costly part is when you start to grow out plants. You need to keep them somewhere!

Click to expand...

That's a lot less than I anticipated. I'll definitely have to give this the 'ole college try sometime.

Obviously I'll try it on cheap plants at first, but cloning the right plant could easily pay for itself and more.

I've done quite a bit of propegation of Coleus at the greenhouse by taking cuttings. The only really important part is getting some sort of antifungal agent on the open cut prior to putting in the medium. For what it's worth, we use Perlite for all of our cutting propegetion and it works very well. Drainage is one of the most important things to have when doing cutting propegation, if the medium stays saturated it will get fungi and all sort of other nasties growing in it.

Just to be clear. There is a difference in propogation from cuttings and micropropogation. Which are you attempting Xema?