Mistergreen, Dr. Loh's video of salt treatment is excellent. It was particularly endearing to me because of the information on using a salt concentration that matches salinity of body fluids so that a weakened fish doesn't have to osmoregulate. I found this to be a key factor in the survival and growth of brine shrimp; harvests were much better at lower salinity [a little greater than 1% (10 ppt)]. That's because if you use higher salinities (e.g., 3.5% or 35 ppt salinity of seawater) the brine shrimp waste precious and considerable energy pumping salt out of their bodies. (Article 'Hatching and Growing Brine Shrimp' discussing this little tidbit is on my
website.)
With sick fish, Dr. Loh in video recommends a similar salt concentration (0.9%) where fish are in an isotonic solution. That is, the water salinity matches the fish's internal salinity. That is exactly the salt concentration (0.9%) that I used to successfully treat my guppies for flukes. However, the treatment took 2 days in a hospital tank.
Based on Dr. Loh's video and guppy resistance to salt, it sounds like jfmary could try a 5 min salt bath- 5 min in a solution of 20-25 g/l. I weighed out a tsp of table salt to get 7 g. So you could add 3 tsp (21 g) of salt to a liter of water. Remember that the smaller the fish, the more they will be stressed by this, so treat the bigger guppies first.
I would use this as an opportunity to weed out your more disease-susceptible guppies- genetically speaking. That's what I do with mine. The only disease I get now is flukes occasionally in juvenile females. I discard the sickest and keep the less affected. These non-sick ones go on fine without further ado to become future breeding stock.
Also, female guppies are more susceptible to flukes--and probably a contagious disease like columnaris--because females socialize and shoal together. That probably explains why you see less of a problem in your male guppies.