First, I am wondering if the CO2 concentration in the water column (in low tech tank), which is being provided by the organic material in the substrate is at an elevated level? This would be as compared to an inert substrate tank with filtration and no injection. Or does that CO2 come out as depleted by the plants in order to maintain an equilibrium? I am wondering this because I want to circulate a low tech planted tank with an airstone and wanted to make sure that it would not deplete the substrate at an accelerated rate. I have heard that it was fine, so I am not too worried about it, but I also wanted to understand the CO2 process with regard to these processes.